Figure 2From: Early differentiation patterning of mouse embryonic stem cells in response to variations in alginate substrate stiffnessRepresentative image of cell morphology of ES-D3 cells during spontaneous differentiation on alginate gels of varying Young’s modulus. Cells on softer alginate gels (516 Pa, Figure 3A) were generally more numerous, but smaller after 3 days of spontaneous differentiation, while cells on stiffer substrates (1337 Pa, Figure 3B) formed larger clumps in a shorter time frame.Back to article page