Table 5 Primer Sequences used for RT-PCR
Gene | Sequence | Gene | Sequence |
|---|---|---|---|
Î’-actin | R 5-tgg gag ggt gag gga ctt-3 | NEST | R 5-ttc ccg tct gct ctg gtt-3 |
L 5-cag cag ttg gtt gga gca-3 | L 5-gga gga tgt ggt gga gga-3 | ||
FGF8 | R 5-tga agg gcg ggtagt tga-3 | REX1 | R 5-tgg gag tca tcg ctt ggt-3 |
L 5-acg gca aag gca agg act-3 | L 5-aag gtc atc cac ggc aca-3 | ||
GSC | R 5-tcg ctt ctg tcg tct cga-3 | CXCR4 | R 5-atg acc agg atc acc aat cca-3 |
L 5-gca ccg cac cat ctt ca-3 | L 5-cgg gat gaa aac gtc cat tt-3 | ||
BMP4 | R 5-cgc tcc gaa tgg cac ta-3 | TTR | R 5-ggc aag atc ctg gtc ctc ct-3 |
L 5-atc tgg tct ccg tcc ctg a-3 | L 5-ttc aca gcc aac gac tct gg-3 | ||
FGF5 | R 5-tag gca cag cag agg gat g-3 | FOXA3 | R 5-gcc cag tag gag cct ttg cc-3 |
L 5-ttc aag cag tcc gag caa-3 | L 5-cgg gcg agg tgt att ctc ca-3 | ||
OCT4 | R 5-gct gat tgg cga tgt gag-3 | HEX | R 5-tca gaa gag ctg tgg tta acc aa-3 |
L 5-gga gaa gtg ggt gga gga a-3 | L 5-agg ccg agt gtg aat cag ag-3 | ||
FOXA2 | R 5-cgc cca cat agg atg aca tg-3 | CLDN | R 5-tca caca ta att ctt ggt ggg a-3 |
L 5-atg gcc tct act ggt ctg ca-3 | |||
L 5-gtt aaa gta tgc tgg gag ccg -3 | |||
HNF4 | R 5-ccc tca gca cac ggt ttt-3 | HNF1B | R 5-gga act ctg ata caa cac cag gct-3 |
L 5-cat cgt caa gcc tcc ctc t-3 | L 5-gcc tcc act cag gca cag agc-3 | ||
AFP | R 5-aac tgg aag ggt ggg aca-3 | GATA6 | R 5-ctc ttg gta gca cca gct ca-3 |
L 5-ctc tgg cga tgg gtg ttt-3 | L 5-gca atg cat gcg gtc tct ac-3 | ||
SOX17 | R 5-aca cca cgg aggaaa tgg-3 | GATA4 | R 5-gag cag gag gca gac aag a-3 |
L 5-atc caa cca gcc cac tga-3 | L 5-gag caa ccg caa atc caa-3 | ||
BRACH | R 5-gcg agt ctg ggt gga tgt a-3 | SOX2 | R 5-ttg gat ggg att ggt ggt-3 |
| Â | L 5-aag aac ggc agg agg atg-3 | Â | L 5-ctg gac tgc gaa ctg gag a-3 |