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Table 1 Gene sequences for mRNA expression during chondrogenesis

From: A fibrin/hyaluronic acid hydrogel for the delivery of mesenchymal stem cells and potential for articular cartilage repair

Chondrogenic marker

Symbols

mRNA Sequence

  

Left primer

Right primer

Glyceraldehyde-3-Phosphate Dehydrogenase†

GAPDH

tagtagccgggccctacttt

tcctcctgtttcatccaagc

Aggrecan

ACAN

cctccccttcacgtgtaaaa

gctccgcttctgtagtctgc

Collagen Type 1 Alpha 1

COL1A1

gggattccctggacctaaag

ggaacacctcgctctcca

Collagen Type 2 Alpha 1

COL2A1

agggccaggatgtccatt

aggagagggcccacagag

SRY-box containing gene 9

SOX9

gtacccgcacttgcacaac

tctcgctctcgttcagaagtc

  1. † - Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is the housekeeping gene.
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Journal of Biological Engineering

ISSN: 1754-1611