Figure 2

Elution profile of IgG on affinity columns with immobilized 4 × PAZ variants. (a) Elution profile of IgG from affinity columns prepared with immobilized 4 × PAZ03. IgG was eluted with pH 5.0, pH 5.5 or pH 6.0 buffer, (b) Elution profile of IgG from affinity columns prepared with immobilized 4 × PAZ01 or rProteinA (GE Healthcare). Elution was performed using pH 5.0 buffer. Affinity columns were prepared using the 4 × PAZ variants. The captured IgG on the column was eluted using a buffer with the pH indicated in the Figure. Afterwards, the residual IgG was eluted with a decreasing pH gradient. The ordinate on the right indicates the pH of the solution. The ordinate on the left indicates the absorbance at 280 nm (mAU: milliabsorbance units). The abscissa indicates the elution volume. The solid and dashed lines show the elution patterns of IgG on 4 × PAZ variants or rProteinA (GE Healthcare) columns, as shown in the Figure, and the pH value of the elution buffer, respectively.