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Figure 1 | Journal of Biological Engineering

Figure 1

From: “NiCo Buster”: engineering E. coli for fast and efficient capture of cobalt and nickel

Figure 1

Schematic representation of the engineered plasmid conferring constitutive metal uptake and inducible adherence. Two synthetic operons were designed and introduced in the pSB1T3 plasmid to generate pIG50 (Table 1). The first operon controls metal uptake by placing the codon-optimized nickel and cobalt transporter gene from Novosphingobium aromaticivorans nicoTB under the control of the strong promoter Ptac. The second operon confers metal-inducible adherence due to genes encoding curli structural (CsgA, CsgB) and assembly (CsgE, CsgF, CsgG) proteins under the control of the nickel and cobalt sensitive promoter from E. coli, Prcn. The bidirectional terminator Bba_B0014 [37] was added to prevent read-through transcription Ptac, nicoTB-BBa_B0014 and rcn-csgBAEFG were obtained separately by direct synthesis and were assembled in the high-copy vector pSB1T3.

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