Table 1 Bacterial strains and plasmids used in this study
From: “NiCo Buster”: engineering E. coli for fast and efficient capture of cobalt and nickel
Strain | Relevant description | Reference |
|---|---|---|
ARY023 | rcnA::uidA-kan | [16] |
HYD720 | ΔnikA-kan | [24] |
MG1655 | F- λ- | |
S57 | MG1655/pIG50 | this study |
S59 | MG1655 nikA | this study |
S63 | S59/pIG50 | this study |
SCC1 = S29 | MG1655 (PA1/04/03 gfpmut3*Cm) | [25] |
S48 | MG1655 (PA1/04/03 gfpmut3*Cm) rcnA::uidA-kan, | this study |
S61 “NiCo buster” | S48/pIG50 | this study |
1137 = S71 | MG1655 malT::Tn 10 ompR234 csgA::uidA-kan | [26] |
Plasmids | Relevant description | Reference |
pSB1C3 | pUC19-derived pMB1 (copy number: 100–300) CmR | [27] |
pSB1T3 | pUC19-derived pMB1 (copy number: 100–300) TetR | [28] |
pIG2 | rcn-csgBAEFG inserted at sites Eco RI/Pst I of pUC57 | [22] |
pIG49 | Ptac-nicoTB in pSB1C3 | this study |
pIG50 | Ptac-nicoTB-rcn-csgBAEFG in pSB1T3 | this study |