Figure 3
From: Challenges in predicting the evolutionary maintenance of a phage transgene
Assays of dispersin activity using a colorimetric substrate. Note that a high absorbance in this assay indicates high enzyme activity (in contrast to the CV assay). The Pure enzyme is a commercial preparation and shows high activity, reflecting its activity as an exoglycosidase on a substrate designed for that activity. The control is from a freeze-thaw lysate of BL21(DE3) cells carrying an empty pET15b plasmid. The bars for enzyme extract (Xtr) are from freeze-thaw lysates of BL21(DE3) cells carrying plasmid pET15b-dspB, with the volume of the extract in parentheses. Bars of 1 std. error are shown (sometimes too small to view).