Figure 4

Dispersin enzyme activity on short term biofilms without added phage (left panel) or with T7v (right panel). After 5 hr of enzyme (with or without concurrent phage treatment), the biofilm wells were stained with crystal violet, and absorbance was measured on the eluted wash. A strong effect of enzyme is observed, and phage addition had no significant effect (no pairwise t-tests were significant). The enzyme-free control (None) is leftmost in each panel, followed by the pure, commercial enzyme (Pure), and the 3–4 rightmost histograms are for different volumes of dispersin extract (Xtr), the same as used in Figure 3. The effect of 0.01 μ L of extract is shown in the presence of phage primarily to demonstrate that the phage were not having an effect that was masked by enzyme. Error bars indicate 1 std error.