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Table 2 Phage evolution in long term biofilms 1

From: Challenges in predicting the evolutionary maintenance of a phage transgene

Initial phages2

Final dsp frequency3

Confidence interval4

T7dsp

1.0

(0.94, 1)

T7dsp

1.0

(0.94, 1)

T7dsp, T7v

0.15

(0.07, 0.33)

T7dsp, T7v

0.0

(0, 0.06)

T7dsp, T7+

0.5

(0.24, 0.76)

T7dsp, T7+

0.15

(0.07, 0.33)

  1. 1The biofilms assayed here are a subset of those shown in Figures 5 and 6. 2T7dsp is T7dsp+trx 10B. Where two phages were inoculated, the starting frequencies were approximately 0.5 each. 3After 96hr phage growth, frequencies were estimated from 8 PCR reactions per sample for each of two primer sets, each reaction comprised of 6 plaque isolates. The PCR product from one primer set indicated presence of the dispersin gene in at least one of the 6 isolates of the reaction; the product from the other set indicated its absence in at least one of the 6 isolates. Frequency was estimated as the maximum likelihood value of the corresponding multinomial term. 4The confidence interval was calculated as the Bayesian credible interval using a uniform prior.