Figure 5
Cultivation of K. lactis GG799 expressing the fluorescent protein GFP applying a temperature profile in a MTP. (A) Cultivation and online monitoring of microbial growth (via scattered light) without induction in complex YP medium containing 20 g/L glucose. (B) Cultivation and online monitoring of microbial growth and (C) fluorescent protein formation in complex YP medium containing 20 g/L galactose as substrate and inducer. Culture conditions: 96well MTP, VL = 200 μL, n = 995 rpm, d0 = 3 mm, aeration with 100% oxygen. Temperature profile: RT = 30°C, Tset,low = 5°C, Tset,high = 50°C (comp. Figure 3A). Data of 12 (from 48) exemplary wells.