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Figure 8 | Journal of Biological Engineering

Figure 8

From: Minireactor-based high-throughput temperature profiling for the optimization of microbial and enzymatic processes

Figure 8

Cultivations of E.coli BL21 expressing the recombinant enzyme ADH-A applying 2 temperature profiles in MTPs. (A) Cultivation and online monitoring of microbial growth (via scattered light) in complex auto-induction medium OnEx. (B) Temperature dependent volumetric activity at the end of the cultivation in the MTP. Reference values from additional cultivations in shake flasks at different temperatures. Culture conditions: 96well MTP, VL = 200 μL, n = 995 rpm, d0 = 3 mm, aeration with 100% oxygen. Temperature profile I for T = 32.2-46.3°C: RT = 37°C, Tset,low = 5°C, Tset,high = 60°C (comp. Figure 3D); Temperature profile II for T = 18.1-28,5°C: RT = 30°C, Tset,low = 5°C, Tset,high = 40°C (comp. Figure 3B). Reference cultivation: 250 ml shake flask, VL = 10 mL, n = 350 rpm, d0 = 50 mm, aeration with air. Online data of 10 exemplary wells.

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