Figure 3

Tetracycline-induced cell death in Casp2 -engineered T-REx-293 cells. (a) Uninduced cells from clone THAP2-2 (a representative clone of T-REx-293 cells carrying the elective cell death module) showed normal growth, but extensive cell death when induced with tetracycline (1 μg/mL) for 48 h. (b) Numbers of adherent, uninduced and induced THAP2-2 cells were monitored over 48 h after induction in 24-well plates. Standard deviation bars: n = 3. (c) When medium was supplemented with caspase inhibitor Q-VD-OPh (50 μM), cell death was largely blocked. Graph: percentages of area covered by adherent cells in various image fields: under normal growth conditions, after tetracycline induction, after tetracycline induction in the presence of Q-VD-OPh or in the presence of Q-V-D-OPh alone. Scale bars: 100 μm.