Immunofluorescence analysis of NPCs differentiated on plain, siNEG PD-fiber and siREST PD-fiber for 7 days. (A) Immunostaining for Tuj1 (early neuronal marker, red), Map2 (late neuronal marker, green), GFAP (glial marker, yellow) and O4 (glial marker, yellow). Nuclei were counterstained with DAPI (blue). (B) Quantification of immunostaining results showing percentage positive staining of various neural and glial cell markers for NPCs cultured on all PD-fiber samples. *indicates p < 0.05 (ANOVA). # and ## indicate p < 0.05 and p < 0.01 (ANOVA) when PD-fiber samples were compared to respective PD-film samples. Mean ± SE, n = 3 , Copyright 2013. Reproduced with permission from Elsevier.