Half-life measurements of chemical inducers for recombinant gene expression

Politi, Nicolo’; Pasotti, Lorenzo; Zucca, Susanna; Casanova, Michela; Micoli, Giuseppina; Cusella De Angelis, Maria Gabriella; Magni, Paolo

doi:10.1186/1754-1611-8-5

Journal of Biological Engineering

Table 1 Degradation rate measurements, expressed as h ^-1 , for IPTG, ATc and HSL in all the tested conditions

From: Half-life measurements of chemical inducers for recombinant gene expression

pH	Temperature	Sterility	Medium	k (L; U), IPTG	k (L; U), ATc	k (L; U), HSL
6	30°C	Sterile	LB	0.001	0.021	0.001
6	30°C	Sterile	LB	(−0.002; 0.005)	(0.018; 0.024)	(−0.001; 0.003)
6	30°C	Sterile	M9	−0.005	0.029	0.021
6	30°C	Sterile	M9	(−0.017; 0.007)	(0.022; 0.035)	(0.012; 0.029)
6	37°C	Sterile	LB	0.001	0.039	0.01
6	37°C	Sterile	LB	(−0.002; 0.004)	(0.03; 0.049)	(0.007; 0.014)
6	37°C	Sterile	M9	−0.002	0.044	0.034
6	37°C	Sterile	M9	(−0.01; 0.006)	(0.042; 0.047)	(0.024; 0.044)
7	30°C	Sterile	LB	0.005	0.017	0.019
7	30°C	Sterile	LB	(0; 0.01)	(0.016; 0.019)	(0.013; 0.024)
7	30°C	Sterile	M9	−0.001	0.021	0.085
7	30°C	Sterile	M9	(−0.005; 0.004)	(0.012; 0.031)	(0.069; 0.102)
7	37°C	Sterile	LB	−0.004	0.029	0.039
7	37°C	Sterile	LB	(−0.011; 0.002)	(0.019; 0.038)	(0.037; 0.042)
7	37°C	Sterile	M9	−0.003	0.041	0.076
7	37°C	Sterile	M9	(−0.01; 0.003)	(0.036; 0.046)	(0.037; 0.114)
6	30°C	Cultured	LB	0.116	0.021	−0.006
6	30°C	Cultured	LB	(0.09; 0.143)	(0.007; 0.035)	(−0.009; -0.003)
6	30°C	Cultured	M9	0.037	0.026	0.007
6	30°C	Cultured	M9	(0.027; 0.048)	(0.018; 0.033)	(0.002; 0.012)
6	37°C	Cultured	LB	0.115	0.051	0.027
6	37°C	Cultured	LB	(0.089; 0.141)	(0.035; 0.067)	(0.013; 0.04)
6	37°C	Cultured	M9	0.114	0.053	0.013
6	37°C	Cultured	M9	(0.07; 0.158)	(0.032; 0.073)	(0.003; 0.024)
7	30°C	Cultured	LB	0.096	0.02	0.063
7	30°C	Cultured	LB	(0.072; 0.12)	(0.006; 0.033)	(0.032; 0.094)
7	30°C	Cultured	M9	0.096	0.031	0.062
7	30°C	Cultured	M9	(0.069; 0.122)	(0.011; 0.052)	(0.045; 0.079)
7	37°C	Cultured	LB	0.075	0.042	0.091
7	37°C	Cultured	LB	(0.024; 0.126)	(0.021; 0.064)	(0.066; 0.116)
7	37°C	Cultured	M9	0.093	0.058	0.088
7	37°C	Cultured	M9	(0.071; 0.114)	(0.038; 0.079)	(0.062; 0.114)

Estimated lower (L) and upper (U) 95% confidence intervals of the measured decay rates (k) are also reported. According to the first-order decay model (see Methods), negative values of k are due to experimental noise and indicate a non-significant decaying trend of the inducer. In this table, cultured broth conditions were tested with the MG1655-Z1 strain.

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ISSN: 1754-1611

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