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Table 1 Degradation rate measurements, expressed as h -1 , for IPTG, ATc and HSL in all the tested conditions

From: Half-life measurements of chemical inducers for recombinant gene expression

pH Temperature Sterility Medium k (L; U), IPTG k (L; U), ATc k (L; U), HSL
6 30°C Sterile LB 0.001 0.021 0.001
(−0.002; 0.005) (0.018; 0.024) (−0.001; 0.003)
6 30°C Sterile M9 −0.005 0.029 0.021
(−0.017; 0.007) (0.022; 0.035) (0.012; 0.029)
6 37°C Sterile LB 0.001 0.039 0.01
(−0.002; 0.004) (0.03; 0.049) (0.007; 0.014)
6 37°C Sterile M9 −0.002 0.044 0.034
(−0.01; 0.006) (0.042; 0.047) (0.024; 0.044)
7 30°C Sterile LB 0.005 0.017 0.019
(0; 0.01) (0.016; 0.019) (0.013; 0.024)
7 30°C Sterile M9 −0.001 0.021 0.085
(−0.005; 0.004) (0.012; 0.031) (0.069; 0.102)
7 37°C Sterile LB −0.004 0.029 0.039
(−0.011; 0.002) (0.019; 0.038) (0.037; 0.042)
7 37°C Sterile M9 −0.003 0.041 0.076
(−0.01; 0.003) (0.036; 0.046) (0.037; 0.114)
6 30°C Cultured LB 0.116 0.021 −0.006
(0.09; 0.143) (0.007; 0.035) (−0.009; -0.003)
6 30°C Cultured M9 0.037 0.026 0.007
(0.027; 0.048) (0.018; 0.033) (0.002; 0.012)
6 37°C Cultured LB 0.115 0.051 0.027
(0.089; 0.141) (0.035; 0.067) (0.013; 0.04)
6 37°C Cultured M9 0.114 0.053 0.013
(0.07; 0.158) (0.032; 0.073) (0.003; 0.024)
7 30°C Cultured LB 0.096 0.02 0.063
(0.072; 0.12) (0.006; 0.033) (0.032; 0.094)
7 30°C Cultured M9 0.096 0.031 0.062
(0.069; 0.122) (0.011; 0.052) (0.045; 0.079)
7 37°C Cultured LB 0.075 0.042 0.091
(0.024; 0.126) (0.021; 0.064) (0.066; 0.116)
7 37°C Cultured M9 0.093 0.058 0.088
(0.071; 0.114) (0.038; 0.079) (0.062; 0.114)
  1. Estimated lower (L) and upper (U) 95% confidence intervals of the measured decay rates (k) are also reported. According to the first-order decay model (see Methods), negative values of k are due to experimental noise and indicate a non-significant decaying trend of the inducer. In this table, cultured broth conditions were tested with the MG1655-Z1 strain.