From: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering
Application | E. colistrain |
---|---|
Transformation of large plasmids | GeneHogs, XL10 Gold, STBL4, SURE |
Generation of single-stranded (ssDNA) | INV110, JM109, JS5, NM522, SCS110, SURE, STBL4, XL10 Gold, XL1 Blue, TG1 |
For storage of plasmids that tend to recombine | DH10B, DH5α, STBL2, STBL3, STBL4, SURE, GeneHogs, Hb101, JM109, JS5, XL10 Gold, XL1 Blue, GC5, GC10 |
Rapid cloning (fast cell growth) | Mach1 |
Cloning of unstable plasmids | SURE, STBL2, STBL3, STBL4 |
High efficiency cloning for library construction | XL10-Gold, MegaX, DH10B |
Blue/white screening | DH10B, DH5α, MC1061, TOP10, XL1 Blue, Hb101, NM522, SCS110, STBL4, SURE, XL10 Gold, GeneHogs, INV110, JM109, JS5, GC5, GC10 |
For site-directed mutagenesis | XL-mutS, BMH 71–18 mutS, ES1301 mutS |
For random mutagenesis | XL1-Red |
For expression of toxic genes | ABLE C, ABLE K |
General cloning and storage of plasmids | DH10B, DH5α, MC1061, TOP10, XL1-Blue, Hb101 |
For proliferation of plasmids encoding the ccdB toxic gene (important in Gateway cloning) | ccdB survival, DB3.1, XL1 Blue, JM109, DH5α |
For generation of unmethylated DNA to be cut with methylation-sensitive restriction enzymes | JM110, ER2925, INV110, DM1, SCS110, E4109S |