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Table 1 Common E. coli host strains in gene cloning

From: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering

Application E. colistrain
Transformation of large plasmids GeneHogs, XL10 Gold, STBL4, SURE
Generation of single-stranded (ssDNA) INV110, JM109, JS5, NM522, SCS110, SURE, STBL4, XL10 Gold, XL1 Blue, TG1
For storage of plasmids that tend to recombine DH10B, DH5α, STBL2, STBL3, STBL4, SURE, GeneHogs, Hb101, JM109, JS5, XL10 Gold, XL1 Blue, GC5, GC10
Rapid cloning (fast cell growth) Mach1
Cloning of unstable plasmids SURE, STBL2, STBL3, STBL4
High efficiency cloning for library construction XL10-Gold, MegaX, DH10B
Blue/white screening DH10B, DH5α, MC1061, TOP10, XL1 Blue, Hb101, NM522, SCS110, STBL4, SURE, XL10 Gold, GeneHogs, INV110, JM109, JS5, GC5, GC10
For site-directed mutagenesis XL-mutS, BMH 71–18 mutS, ES1301 mutS
For random mutagenesis XL1-Red
For expression of toxic genes ABLE C, ABLE K
General cloning and storage of plasmids DH10B, DH5α, MC1061, TOP10, XL1-Blue, Hb101
For proliferation of plasmids encoding the ccdB toxic gene (important in Gateway cloning) ccdB survival, DB3.1, XL1 Blue, JM109, DH5α
For generation of unmethylated DNA to be cut with methylation-sensitive restriction enzymes JM110, ER2925, INV110, DM1, SCS110, E4109S
  1. Data are derived from enzyme providers’ data sheets, the following website, and this reference [14].