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Table 1 Common E. coli host strains in gene cloning

From: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering

Application

E. colistrain

Transformation of large plasmids

GeneHogs, XL10 Gold, STBL4, SURE

Generation of single-stranded (ssDNA)

INV110, JM109, JS5, NM522, SCS110, SURE, STBL4, XL10 Gold, XL1 Blue, TG1

For storage of plasmids that tend to recombine

DH10B, DH5α, STBL2, STBL3, STBL4, SURE, GeneHogs, Hb101, JM109, JS5, XL10 Gold, XL1 Blue, GC5, GC10

Rapid cloning (fast cell growth)

Mach1

Cloning of unstable plasmids

SURE, STBL2, STBL3, STBL4

High efficiency cloning for library construction

XL10-Gold, MegaX, DH10B

Blue/white screening

DH10B, DH5α, MC1061, TOP10, XL1 Blue, Hb101, NM522, SCS110, STBL4, SURE, XL10 Gold, GeneHogs, INV110, JM109, JS5, GC5, GC10

For site-directed mutagenesis

XL-mutS, BMH 71–18 mutS, ES1301 mutS

For random mutagenesis

XL1-Red

For expression of toxic genes

ABLE C, ABLE K

General cloning and storage of plasmids

DH10B, DH5α, MC1061, TOP10, XL1-Blue, Hb101

For proliferation of plasmids encoding the ccdB toxic gene (important in Gateway cloning)

ccdB survival, DB3.1, XL1 Blue, JM109, DH5α

For generation of unmethylated DNA to be cut with methylation-sensitive restriction enzymes

JM110, ER2925, INV110, DM1, SCS110, E4109S

  1. Data are derived from enzyme providers’ data sheets, the following website, and this reference [14]. http://openwetware.org/wiki/E._coli_genotypes.