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Table 2 Common vectors in gene cloning

From: Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering

Plasmid name Advantages Disadvantages References
pBR322 Small size (4.4 kb), variety of cloning sites, medium copy number (15–20)   [24, 25]
pUC18 and pUC19 Small size (2.7 kb), high copy number (500–700), multiple cloning site, sequencing using M13 primers Not good if target protein is toxic or for membrane proteins, needs blue/white screen [24, 26]
pLG338 General purpose plasmid vector, size 7.3 kb, low copy number (6–8), genes coding for membrane and regulatory proteins which cannot be cloned into high-copy-number plasmids   [27]
pMiniT Inserted PCR product disrupts a toxic minigene, no blue/white selection required, works for both blunt end and single-base overhang-containing PCR products   NEB
pCR™4Blunt-TOPO® Inserted PCR product disrupts ccdB toxic gene [28], works based on topoisomerase I [29], no blue/white selection required Needs blunt-end PCR products Life Technologies
pDrive Size 3.8 kb Needs single A overhangs, not usable for proofreading DNA polymerases, needs blue/white screening QIAGEN
StrataClone Blunt PCR Cloning Vector pSC-B-amp/kan Uses the DNA topoisomerase I and the DNA recombination activity of Cre recombinase, up to 9 kb PCR product size Needs blunt-end PCR products, needs special competent cells for transformation (cells expressing Cre recombinase) Agilent Technologies
pJET1.2/blunt Inserted PCR product disrupts the toxic gene eco47IR, no need for blue/white screen, works for both blunt end and single-base overhang-containing PCR products, up to 10 kb PCR product size   Thermo Scientific
  1. Data are derived from vector providers or the cited references. pBR322, pUC18, pUC19, and pLG338 are cloning vectors and the rest are PCR sequencing vectors.