Figure 3

Microscopy image of the buckled Dictyostelium lipid patch after injection of the cytosol extract of Dictyostelium cells. 3D-merged reconstructed images of the autofluorescence images of a typical Dictyostelium lipid patch obtained by confocal laser scanning fluorescence microscopy with both Red and Green detection modes before (A) and 30 min after (B) injection of cytosol extract. The volume size of observation space is 210 μm x 210 μm x 37.5 μm. Each x-z plane cross-section of the 3D merged reconstructed image was attached in the right column. Dashed lines correspond to the surface of the glass slide. The height indicates the distance between the surface of the glass slide and the peak top position of the autofluorescence images.