Figure 4

Microscopy image of the buckled Dictyostelium lipid patch after injection of the cytosol extract of PH-crac-RFP/PTEN-GFP co-expressing cells. 3D merge of reconstructed fluorescence images of a typical patch of the Dictyostelium lipid membrane film obtained by confocal laser scanning fluorescence microscopy, with both Red and Green fluorescence filter units, before injection of cytosol extract. (A) 3D merge of reconstructed fluorescence images of a typical patch of the Dictyostelium lipid membrane film obtained by confocal laser scanning fluorescence microscopy, with both Red and Green fluorescence filter units, before injection of cytosol extract. The volume size of observation space is 210 μm x 210 μm x 37.5 μm. The mean fluorescence intensity of each Red and Green fluorescence image was plotted along the z position in (D) and (E) respectively. (B) 3D merge of reconstructed fluorescence images and (C) the cross-section reconstructed fluorescence images of a typical patch of the lipid membrane film obtained by confocal laser scanning fluorescence microscopy with both Red and Green fluorescence filter units, 30 min after injection of the cytosol extract of PH-crac-RFP/PTEN-GFP co-expressing cells. The mean fluorescence intensity of each Red and Green fluorescence image was plotted along z position in (F) and (G), respectively. The height shown in (A-C) indicates the distance between the surface of the glass slide and the peak top position of the autofluorescence images.