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Figure 5 | Journal of Biological Engineering

Figure 5

From: Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum

Figure 5

Time course change of fluorescence intensity of the Dictyostelium lipid patch. Time course change of the mean fluorescence intensity analyzed by fluorescence images (z position; two image frames (~1 μm) above the glass slide) of the Dictyostelium lipid patch obtained by confocal laser scanning fluorescence microscopy images with both Red (A) and Green (B) fluorescence detection filter units after injection of the cytosol extract of PH-crac-RFP/PTEN-GFP co-expressing cells. The slope in the range of 30 – 180 s is depicted in each diagram. (C) Time course change of the mean fluorescence intensity analyzed by fluorescence images (z position; two image frames (~1 μm) above the glass slide) of a typical patch of the POPC/PIP3 membrane film obtained by confocal laser scanning fluorescence microscopy images with Green fluorescence detection filter units after injection of the cytosol extract of PH-crac-RFP/PTEN-GFP co-expressing cells.

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Journal of Biological Engineering

ISSN: 1754-1611