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Figure 3 | Journal of Biological Engineering

Figure 3

From: Synthetic feedback control using an RNAi-based gene-regulatory device

Figure 3

Parameters that affect performance of the RNAi-based OFF control device. (a-c) Predicted relationships between device dynamic range and the length of the spacer between the ribozyme switch and miRNA components (a), miRNA-target transcript binding affinity, K m (b), and the ratio of ribozyme cleavage rates in the absence and presence of ligand, k cleave /k cleave-bound (c). Grey line: model predictions; circle: experimental results. Device dynamic range is reported as the percent change in GFP expression in the absence and presence of theophylline.

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