Skip to main content

Table 1 EGFR binding affinity parameters for yeast-displayed wild type CA and His variants

From: Engineering pH responsive fibronectin domains for biomedical applications

Fn3 Domain

KD pH 5.5 (M)

KD pH 7.4 (M)

KD Ratio (5.5/7.4)

Wild Type Clone A

(2.4 +/− 0.4) •10−9

(1.7 +/− 0.1) •10−9

1.4 +/− 0.2

F48H

(1.1 +/− 0.4) •10−8

(4.8 +/− 0.8) •10−9

2.3 +/− 0.4

T49H

(8 +/− 2) •10−9

(1.3 +/− 0.5) •10−9

6.3 +/− 0.7

F48H/T49H

(1.5 +/− 0.4) •10−8

(3 +/− 1) •10−9

5.2 +/− 0.4

  1. Error bars denote standard deviations for two independent trials in which KDs were determined by fitting data obtained in respective binding affinity titrations conducted at pH 5.5 and pH 7.4 and carried out simultaneously. For each clone, two independent KD ratio values were obtained by dividing the KD values that were calculated based on the data for each of the two independent pairs of EGFR binding affinity titrations