From: Engineering pH responsive fibronectin domains for biomedical applications
Fn3 Domain | KD pHÂ 5.5 (M) | KD pHÂ 7.4 (M) | KD Ratio (5.5/7.4) |
---|---|---|---|
Wild Type Clone A | (2.4 +/− 0.4) •10−9 | (1.7 +/− 0.1) •10−9 | 1.4 +/− 0.2 |
F48H | (1.1 +/− 0.4) •10−8 | (4.8 +/− 0.8) •10−9 | 2.3 +/− 0.4 |
T49H | (8 +/− 2) •10−9 | (1.3 +/− 0.5) •10−9 | 6.3 +/− 0.7 |
F48H/T49H | (1.5 +/− 0.4) •10−8 | (3 +/− 1) •10−9 | 5.2 +/− 0.4 |