Fig. 4

Online and offline data from parallel fermentations of E. coli BL21 EcFbFP under non-oxygen-limited and oxygen-limited conditions. Cultivation without oxygen limitation (a, c, e) in WilmsMOPS medium supplemented with 10 g/L glucose, buffered with 0.2 M MOPS. Culture conditions for RAMOS: 250 mL RAMOS shake flasks, V_L = 8 mL, n = 350 rpm, d₀ = 50 mm, T = 37 °C. Culture conditions for BioLector: 48-well Flowerplate, V_L = 1 mL, n = 1500 rpm, d₀ = 3 mm, T = 37 °C. Cultivation under oxygen-limited conditions (b, d, f) in WilmsMOPS medium supplemented with 15 g/L glucose, buffered with 0.2 M MOPS. Culture conditions for RAMOS: 250 mL RAMOS shake flasks, V_L = 25 mL, n = 350 rpm, d₀ = 50 mm, T = 37 °C. Culture conditions for BioLector: 48-well Flowerplate, V_L = 1 mL, n = 800 rpm, d₀ = 3 mm, T = 37 °C. For better visibility, product fluorescence values in (e) and (f) were multiplied by a factor of three. OTR_calc is the calculated OTR course for the MTP determined from the Eq. 2. The k_La values for non-oxygen limited and oxygen-limited cultivation were 603 h⁻¹ and 183 h⁻¹, respectively