Fig. 6
Online and offline data from parallel fermentations of E. coli BL21 BSLA in auto-induction medium. Cultivation in WilmsMOPS mineral auto-induction medium containing 0.5 g/L glucose, 2 g/L lactose and 5 g/L glycerol, buffered with 0.2 M MOPS (a-c). Culture conditions for RAMOS: 250 mL RAMOS shake flasks, VL = 10 mL, n = 350 rpm, d0 = 50 mm, T = 37 °C. Culture conditions for BioLector: 48-well Flowerplate, VL = 1 mL, n = 1500 rpm, d0 = 3 mm, T = 37 °C. The kLa value used for calculation of the OTRcalc course was 587 h−1