Skip to main content

Table 1 Overview of excitation and emission wavelengths in the BioLector

From: Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors

Signal

Filter (excitation/emission)

Gain

E.coli BL21 EcFbFP

  

 Scattered light

620 nm/-

30

 FbFP-fluorescence

450 nm/492 nm

60

 pO2-optode

520 nm/600 nm

60

 pH -optode

470 nm/525 nm

45

E.coli BL21 BSLA

  

 Scattered light

620 nm/-

30

 FbFP-fluorescence

450 nm/492 nm

60

 pO2-optode

520 nm/600 nm

60

G. oxydans 3504

  

 Scattered light

620 nm/-

30

 pO2-optode

520 nm/600 nm

50

K. lactis Cel5A

  

 Scattered light

620 nm/-

30

 pO2-optode

505 nm/590 nm

50