Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors

Table 2 Overview of the fitted k_La values

Cultivation	k_La [h⁻¹]	Cultivation temperature [°C]	Shaking parameters
			MTP¹		Shake flask²
			n [rpm]	V_L [mL]	n [rpm]	V_L [mL]
E.coli non-oxygen-limited	603	37	350	8	1500	1
E.coli oxygen-limited	183	37	350	25	800	1
E.coli diauxic growth	276	30	350	12	1000	0.6
E.coli induction	587	37	350	10	1500	1
G. oxydans unbuffered	425	30	350	10	1250	1
G. oxydans buffered	413	30	350	10	1250	1
K. lactis oxygen-limited	203	30	350	20	800	1

ISSN: 1754-1611