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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Chemical lysis of cyanobacteria

Fig. 2

Comparison of cyanophage lysozyme with T4 lysozyme for cyanobacterial lysis. The concentration of cyanophage lysozyme (purified in-house) and T4 lysozyme (MCLab) was titrated to compare their effectiveness in lysing Synechocystis sp. PCC 6803. Each reaction contained lysozyme at the indicated concentration, 25 mM MES buffer (pH 6.2), and 1 X BugBuster reagent. Reactions were incubated for 90 min at 37 °C. We estimated that the maximum lysis achieved here corresponded to 50 % of total lysis; approx. 25 % of the cells were lysed with BugBuster alone. Error bars are standard deviations of triplicate reactions

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