Plasmid | Backbone::insert |
---|
pKM011 | pET21b::cpL1 |
pKM012 | pY71X::cpL1 |
pKM013 | pET21b::cpL2 |
pKM014 | pY71X::cpL2 |
pKM015 | pET21b::cpL3 |
pKM016 | pY71X::cpL3 |
pKM024 | pET21b::strepII-GSA-cpL1 |
pKM025 | pET21b::cpL1-GSA-strepII |
- The lysozyme gene used in this study was “cyanophage lysozyme 1” (cpL1; sequence provided in Additional file 1). Two other genes, cpL2 and cpL3, were also cloned, but did not express solubly in E. coli. The genes were cloned into either the pET21b vector for in vivo expression or the pY71X vector for cell-free expression (data not shown)