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Table 1 Plasmids constructed for this work

From: Chemical lysis of cyanobacteria

Plasmid

Backbone::insert

pKM011

pET21b::cpL1

pKM012

pY71X::cpL1

pKM013

pET21b::cpL2

pKM014

pY71X::cpL2

pKM015

pET21b::cpL3

pKM016

pY71X::cpL3

pKM024

pET21b::strepII-GSA-cpL1

pKM025

pET21b::cpL1-GSA-strepII

  1. The lysozyme gene used in this study was “cyanophage lysozyme 1” (cpL1; sequence provided in Additional file 1). Two other genes, cpL2 and cpL3, were also cloned, but did not express solubly in E. coli. The genes were cloned into either the pET21b vector for in vivo expression or the pY71X vector for cell-free expression (data not shown)