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Table 2 Primer names and sequences used

From: Essential validation methods for E. coli strains created by chromosome engineering

Primer name Primer sequence
PmotABfw 5′- GCTGAAGCCAAAAGTTCCTG-3′
PmotABrv 5′- TGCCTGCAGCTTATGTCAAC-3′
PcmRfw 5′- ATCACAAACGGCATGATGAA-3′
PcmRrv 5′- TCACTACCGGGCGTATTTTT-3′
PgalKfw 5′- TCCATCAGCGTGACTACCATC-3′
PgalKrv 5′- CAGAACAGGCAGCAGAGCGT-3′