Table 2 Primer names and sequences used
From: Essential validation methods for E. coli strains created by chromosome engineering
Primer name | Primer sequence |
|---|---|
PmotABfw | 5′- GCTGAAGCCAAAAGTTCCTG-3′ |
PmotABrv | 5′- TGCCTGCAGCTTATGTCAAC-3′ |
PcmRfw | 5′- ATCACAAACGGCATGATGAA-3′ |
PcmRrv | 5′- TCACTACCGGGCGTATTTTT-3′ |
PgalKfw | 5′- TCCATCAGCGTGACTACCATC-3′ |
PgalKrv | 5′- CAGAACAGGCAGCAGAGCGT-3′ |