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Table 3 Summary of DNA sequence analysis results for various ΔmotAB colonies

From: Essential validation methods for E. coli strains created by chromosome engineering

Colony id

Summary of DNA sequence analysis results

ΔmotAB1

Positive (Good signal at both the integration sites and insert)

ΔmotAB2

Positive (Good signal at both the integration sites and insert)

ΔmotAB3

Negative (Double signal at the end)

ΔmotAB4

Positive (Good signal at both the integration sites and insert)

ΔmotAB5

Positive (Good signal at both the integration sites and insert)

ΔmotAB6

Negative (Low signal)

ΔmotAB7

Negative (Double signal at the end)

ΔmotAB8

Negative (Double signal at the beginning)

ΔmotAB9

Negative (Low signal)

ΔmotAB10

Positive (Good signal at both the integration sites and insert)

ΔmotAB11

Positive (Good signal at both the integration sites and insert)

ΔmotAB12

Negative (Low signal with broad peaks)

ΔmotAB13

Negative (Low signal)

ΔmotAB14

Positive (Good signal at both the integration sites and insert)

ΔmotAB15

Positive (Good signal at both the integration sites and insert)

ΔmotAB16

Positive (Good signal at both the integration sites and insert)