Fig 4

Optimization of megaprimer PCR stage of QuickStep-Cloning. a Yield of megaprimer PCR for varying number of PCR cycles. b Yield of megaprimer PCR for different concentrations of recipient plasmid. c Yield of megaprimer PCR for different concentrations of megaprimer. In all gel pictures, the appearance of low and high molecular weight bands could be attributed to non-specific binding of primers, commonly seen in regular PCRs