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Table 2 Results of rfp cloning experiment

From: QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids

Strain, Selection plate

QuickStep-Cloning

RF Cloning

Transformation efficiency [cfu/μg]

DH5α, Kanamycin

334

26

3.8 · 104

C41(DE3), Kan + IPTG

418(404)

113(103)

4.2 · 106

  1. Colony counts for E. coli strains DH5α and C41 (DE3) transformed with the products of RF cloning and of QuickStep-Cloning and plated on agar plates supplemented with: (i) 50 μg/ml kanamycin and (ii) 50 μg/ml kanamycin and 1 mM IPTG. Transformation efficiency was determined based on concurrent transformation of 1 ng intact pET24a-HLTEV-p53 plasmid. Numbers in the brackets denote RFP-expressing colonies, as determined by visual inspection of the plates