Fig. 5
From: Engineering dynamic cell cycle control with synthetic small molecule-responsive RNA devices
Reliability of cell cycle control systems over time. a Performance of the cell cycle controller for G0/1 (integrated p27-switch controller) over time. Cells harboring the G0/1 cell cycle controller (p27 switch th-A) or a negative control (p27 OFF control) were induced with 0 or 1Â mM theophylline (theo) and the percentage of the cell population in G0/1 was measured via DNA staining and flow cytometry after creation of the cell lines (left of hash marks) and at 1 and 3Â weeks after cell lines were frozen and revived (right of hash marks). b Performance of the cell cycle controller for G2/M (integrated CCNB1m-switch controller) over time. Cells harboring the G2/M cell cycle controller (CCNB1m switch th-A) or a negative control (CCNB1m OFF control) were induced with 0 or 1Â mM theophylline and the percentage of the cell population in G2/M was measured via DNA staining and flow cytometry at 1, 2, 3 and 6Â weeks after creation of the cell lines