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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices

Fig. 4

The morphologies of the C2C12 myoblasts on the PLGA and RGD/PLGA nanofiber matrices. Two-photon excitation fluorescence images of C2C12 myoblasts cultured on a PLGA and (b) RGD/PLGA nanofiber matrices for 3 and 7 days. The cell nuclei were counterstained by DAPI (blue), F-actins were stained by TRITC-labelled phalloidin (red) and RGD-M13 phages in the RGD/PLGA nanofiber matrices were immunostained with the FITC-labelled anti-M13 phage antibody (green). c Quantification of the cell area and aspect ratio at 3 days. The asterisk (*) denotes a significant difference between the PLGA and RGD/PLGA nanofiber matrices, p < 0.05. Quantitative analysis was performed using ImageJ Software

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Journal of Biological Engineering

ISSN: 1754-1611