Fig. 5

Stimulated myogenesis of C2C12 myoblasts on RGD/PLGA nanofiber matrices. Two-photon excitation fluorescence images of the C2C12 myoblasts on (a) PLGA and (b) RGD/PLGA nanofiber matrices without the GO, (c) RGD/PLGA nanofiber matrices with the GO and (d) RGD/PLGA nanofiber matrices in differentiation media (DM). The cell nuclei were counterstained by DAPI (blue), F-actins were stained with TRITC-labelled phalloidin (red) and the myosin heavy chains (MHCs) were stained with FITC-labelled anti-MHC antibody (green). The scale bars are 50 μm. All photographs shown in this figure are representative of six independent experiments with similar results. Quantification of (e) the cell area, MHC-positive area, (f) fusion index, maturation index, and (g) myotube length (1: PLGA matrices, 2: RGD/PLGA nanofiber matrices without the GO, 3: RGD/PLGA nanofiber matrices with the GO, and 4: RGD/PLGA nanofiber matrices in DM). An asterisk (*) denotes a significant difference between PLGA matrices and other groups, p < 0.05. Quantitative analysis was performed using ImageJ Software