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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Fig. 3

The results of individual modulation of the RBSs, coding sequences and gene order by OLMA. a RBS library was constructed using the OLMA method. 10–20 RBSs were chosen for each gene of PanEBI with crtE-I-B order. (b) Coding sequence library was constructed by the OLMA method. CrtEBI from 4 species were assembled as crtE-I-B order with the medium strength RBSs E9_6689, B1_5557, I3_3088 and id1_10974. (c) Gene order library was constructed using the OLMA method. The PanE, PanB and PanI genes were assembled in all six orders. The dynamic range and c-factor of each library were shown in the figures

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