Fig. 4
From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method
OLMA was applied to modulate multiple types of targets in a single assembly step. a A combinatorial library of two types of targets (RBS and gene order), was constructed by the OLMA method. The coding sequences here were PanE, PanB and PanI. (b) A combinatorial library of four RBSs and three coding sequences was constructed using the OLMA method. The gene order here was crtE-I-B. (c) A combinatorial library for varying four RBSs, three coding sequences and the gene order was constructed by the OLMA method. The dynamic range and coverage of lycopene yield were shown in the figures