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Table 1 Construction efficiency of the assembly of LacZ gene using OLMA method

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Plasmids

Number of pieces to assemble

Total colonies(cfu/ug input DNA)

Positive rate (%)

lacZ

1

104

99.9

Z1 + Z2 + Z3

3

103

95.0

Z1 + Z2 + Z3 + Z4

4

500

43.0

Z1 + Z2 + Z3 + Z4 + Z5

5

10

10.0