Skip to main content

Table 1 Construction efficiency of the assembly of LacZ gene using OLMA method

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Plasmids Number of pieces to assemble Total colonies(cfu/ug input DNA) Positive rate (%)
lacZ 1 104 99.9
Z1 + Z2 + Z3 3 103 95.0
Z1 + Z2 + Z3 + Z4 4 500 43.0
Z1 + Z2 + Z3 + Z4 + Z5 5 10 10.0