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Table 2 Oligos synthesized for the optimization of lycopene synthestic pathway. Oligos with 3 different overhangs was synthesized for 3 different assembly orders

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Oligo Sequence Oligo Sequence
Reverse design Forward design
E4_17864-R CCGTCATtacaatttcctcattaattgaaca CrtE_13822 R CCGTCATattcactctcctttctttttaccat
E4_17864-F GTATtgttcaattaatgaggaaattgtaATG CrtE_13822 F GTATatggtaaaaagaaaggagagtgaatATG
B-E4_17864-F GGCCtgttcaattaatgaggaaattgtaATG B-CrtE_13822 F GGCCatggtaaaaagaaaggagagtgaatATG
I-E4_17864-F TTAAtgttcaattaatgaggaaattgtaATG I-CrtE_13822 F TTAAatggtaaaaagaaaggagagtgaatATG
B13_51518-R TATTCATtatttctttcctcctttcctttt CrtB_12823 R: TATTCATctagtatttctcctctttctctaga
B13_51518-F GTATaaaaggaaaggaggaaagaaataATG CrtB_12823 F GTATtctagagaaagaggagaaatactagATG
E-B13_51518-F CAGGaaaaggaaaggaggaaagaaataATG E-CrtB_12823 F CAGGtctagagaaagaggagaaatactagATG
I-B13_51518-F TTAAaaaaggaaaggaggaaagaaataATG I-CrtB_12823 F TTAAtctagagaaagaggagaaatactagATG
I15_28086-R GTTTCATatagttcctcctttcagcaaaa CrtI_18732 R: GTTTCATagaattcctcctctttaatgaattc
I15_28086-F GTATttttgctgaaaggaggaactatATG CrtI_18732 F: GTATgaattcattaaagaggaggaattctATG
E-I15_28086-F CAGGttttgctgaaaggaggaactatATG E-CrtI_18732 F: CAGGgaattcattaaagaggaggaattctATG
B-I15_28086-F GGCCttttgctgaaaggaggaactatATG B-CrtI_18732 F: GGCCgaattcattaaagaggaggaattctATG
id16_135699-R TTTGCATttagggcctccttatgtagc idi_14921 R: TTTGCATattttactcctcttcttaaaagatctttt
E-id16_135699-F CAGGgctacataaggaggccctaaATG E-idi_14921 F: CAGGaaaagatcttttaagaagaggagtaaaatATG
B-id16_135699-F GGCCgctacataaggaggccctaaATG B-idi_14921 F: GGCCaaaagatcttttaagaagaggagtaaaatATG
I-id16_135699-F TTAAgctacataaggaggccctaaATG I-idi_14921 F: TTAAaaaagatcttttaagaagaggagtaaaatATG