Table 2 Oligos synthesized for the optimization of lycopene synthestic pathway. Oligos with 3 different overhangs was synthesized for 3 different assembly orders
From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method
Oligo | Sequence | Oligo | Sequence |
|---|---|---|---|
Reverse design | Forward design | ||
E4_17864-R | CCGTCATtacaatttcctcattaattgaaca | CrtE_13822 R | CCGTCATattcactctcctttctttttaccat |
E4_17864-F | GTATtgttcaattaatgaggaaattgtaATG | CrtE_13822 F | GTATatggtaaaaagaaaggagagtgaatATG |
B-E4_17864-F | GGCCtgttcaattaatgaggaaattgtaATG | B-CrtE_13822 F | GGCCatggtaaaaagaaaggagagtgaatATG |
I-E4_17864-F | TTAAtgttcaattaatgaggaaattgtaATG | I-CrtE_13822 F | TTAAatggtaaaaagaaaggagagtgaatATG |
B13_51518-R | TATTCATtatttctttcctcctttcctttt | CrtB_12823 R: | TATTCATctagtatttctcctctttctctaga |
B13_51518-F | GTATaaaaggaaaggaggaaagaaataATG | CrtB_12823 F | GTATtctagagaaagaggagaaatactagATG |
E-B13_51518-F | CAGGaaaaggaaaggaggaaagaaataATG | E-CrtB_12823 F | CAGGtctagagaaagaggagaaatactagATG |
I-B13_51518-F | TTAAaaaaggaaaggaggaaagaaataATG | I-CrtB_12823 F | TTAAtctagagaaagaggagaaatactagATG |
I15_28086-R | GTTTCATatagttcctcctttcagcaaaa | CrtI_18732 R: | GTTTCATagaattcctcctctttaatgaattc |
I15_28086-F | GTATttttgctgaaaggaggaactatATG | CrtI_18732 F: | GTATgaattcattaaagaggaggaattctATG |
E-I15_28086-F | CAGGttttgctgaaaggaggaactatATG | E-CrtI_18732 F: | CAGGgaattcattaaagaggaggaattctATG |
B-I15_28086-F | GGCCttttgctgaaaggaggaactatATG | B-CrtI_18732 F: | GGCCgaattcattaaagaggaggaattctATG |
id16_135699-R | TTTGCATttagggcctccttatgtagc | idi_14921 R: | TTTGCATattttactcctcttcttaaaagatctttt |
E-id16_135699-F | CAGGgctacataaggaggccctaaATG | E-idi_14921 F: | CAGGaaaagatcttttaagaagaggagtaaaatATG |
B-id16_135699-F | GGCCgctacataaggaggccctaaATG | B-idi_14921 F: | GGCCaaaagatcttttaagaagaggagtaaaatATG |
I-id16_135699-F | TTAAgctacataaggaggccctaaATG | I-idi_14921 F: | TTAAaaaagatcttttaagaagaggagtaaaatATG |