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Table 3 Details of the top10 lycopene reporter

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Name

1

2

3

4

5

6

7

8

Yield

Order

RBS for crtE

RBS for crtI

RBS for crtB

RBS for idi

crtE

crtI

crtB

pLY116

BIE

E3_(1813)

I6_(1137)

B8_(2059)

id12_(3867)

PanE

PanI

PanB

15.17

pLY111

BEI

E13_(13670)

I6_(1137)

B1_(5557)

id16_(135699)

PanE

PanI

PanB

14.69

pLY108

EBI

E9_(6689)

I4_(1630)

B13_(51518)

id3_(5078)

PvaE

PanI

PanB

13.86

pLY101

BEI

E3-(1813)

I10_(3562)

B2_(1147)

id15_(115818)

RspE

PanI

PanB

12.81

pLY106

EBI

E9_(6689)

I10_(3562)

B2_(1147)

id6_(4010)

PvaE

PanI

PanB

12.53

pLY112

BEI

E16_(13174)

I6_(1137)

B11_(25607)

id14_(31121)

PvaE

PanI

RspB

12.39

pLY107

EBI

E9_(6689)

I6_(1137)

B14_(46327)

id15_(115818)

PvaE

PagI

PanB

12.07

pLY103

BIE

E14_(5844)

I15_(28086)

B8_(2059)

id14_(31121)

RspE

PanI

PanB

11.69

pLY109

EIB

E3_(1813)

I6_(1137)

B4_(3088)

id1_(10974)

PanE

PanI

RspB

11.33

pLY102

BEI

E2_(2472)

I11_(340)

B8_(2059)

id12_(3867)

RspE

PanI

PanB

11.29

Control

EIB

(17707)

(10318)

(10793)

(31786)

PanE

PanI

PanB

5.54

  1. *The numbers with in the parenthese are the theoretical strength of each gene acquired by RBS calculator. The RBS sequences of crtEBI and idi of the Control strain are all “aaggagatata”