Table 4 Strains and plasmids used in this study
From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method
Strain/plasmid | Description | Reference |
|---|---|---|
Strains | ||
Trans5α | F−φ80d lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rk −, mk +) supE44λ-thi-1 gyrA96relA1phoA | TransGen Biotech |
DB 3.1 | F− gyrA462endA1Δ(sr1-recA) mcrB mrr hsdS20(rB −, mB −) supE44 ara14 galK2 lacY1 proA2 rpsL20(SmR) xyl-5λ- leu mtl1 | Life technology |
PXIDF | BW25113, â–³pgi, pT5-dxs, pT5-idi, pT5-ispDF | Our lab |
Plasmid | ||
pHD-lacZ1 | lacZ cassette (containing PJ23001, lacZ gene, rrnB Terminator) cloned into pHD, TetR | This study |
pHD-lacZ3.1 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ3.2 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ3.3 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ4.1 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ4.2 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ4.3 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ4.4 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ5.1 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ5.2 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ5.3 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ5.4 | Partial lacZ cassette cloned into pHD | This study |
pHD-lacZ5.5 | Partial lacZ cassette cloned into pHD | This study |
pFUS | pUC origin, Spe R, ccdB operon, | This study |
pHD-PanE | crtE from Pantoea ananatis cloned into pHD | This study |
pHD-PagE | crtE from Pantoea agglomerans cloned into pHD | This study |
pHD-PvaE | crtE from Pantoea vagans cloned into pHD | This study |
pHD-RspE | crtE from Rhodobacter sphaeroides cloned into pHD | This study |
pHD-PanB | crtB from Pantoea ananatis cloned into pHD | This study |
pHD-PagB | crtB from Pantoea agglomerans cloned into pHD | This study |
pHD-PvaB | crtB from Pantoea vagans cloned into pHD | This study |
pHD-RspB | crtB from Rhodobacter sphaeroides cloned into pHD | This study |
pHD-PanI | crtI from Pantoea ananatis cloned into pHD | This study |
pHD-PagI | crtI from Pantoea agglomerans cloned into pHD | This study |
pHD-PvaI | crtI from Pantoea vagans cloned into pHD | This study |
pYC1k-ccdB-idi | p15A origin, Tac promoter, KanR, ccdB operon, idi | This study |
pSB1s-X | pSC101 origin, Pbad promoter, StrR, dxs from Escherichia coli cloned into pSB1s | Our lab |