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Table 4 Strains and plasmids used in this study

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Strain/plasmid Description Reference
Strains   
Trans5α Fφ80d lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17 (rk , mk +) supE44λ-thi-1 gyrA96relA1phoA TransGen Biotech
DB 3.1 F gyrA462endA1Δ(sr1-recA) mcrB mrr hsdS20(rB , mB ) supE44 ara14 galK2 lacY1 proA2 rpsL20(SmR) xyl-5λ- leu mtl1 Life technology
PXIDF BW25113, pgi, pT5-dxs, pT5-idi, pT5-ispDF Our lab
Plasmid   
pHD-lacZ1 lacZ cassette (containing PJ23001, lacZ gene, rrnB Terminator) cloned into pHD, TetR This study
pHD-lacZ3.1 Partial lacZ cassette cloned into pHD This study
pHD-lacZ3.2 Partial lacZ cassette cloned into pHD This study
pHD-lacZ3.3 Partial lacZ cassette cloned into pHD This study
pHD-lacZ4.1 Partial lacZ cassette cloned into pHD This study
pHD-lacZ4.2 Partial lacZ cassette cloned into pHD This study
pHD-lacZ4.3 Partial lacZ cassette cloned into pHD This study
pHD-lacZ4.4 Partial lacZ cassette cloned into pHD This study
pHD-lacZ5.1 Partial lacZ cassette cloned into pHD This study
pHD-lacZ5.2 Partial lacZ cassette cloned into pHD This study
pHD-lacZ5.3 Partial lacZ cassette cloned into pHD This study
pHD-lacZ5.4 Partial lacZ cassette cloned into pHD This study
pHD-lacZ5.5 Partial lacZ cassette cloned into pHD This study
pFUS pUC origin, Spe R, ccdB operon, This study
pHD-PanE crtE from Pantoea ananatis cloned into pHD This study
pHD-PagE crtE from Pantoea agglomerans cloned into pHD This study
pHD-PvaE crtE from Pantoea vagans cloned into pHD This study
pHD-RspE crtE from Rhodobacter sphaeroides cloned into pHD This study
pHD-PanB crtB from Pantoea ananatis cloned into pHD This study
pHD-PagB crtB from Pantoea agglomerans cloned into pHD This study
pHD-PvaB crtB from Pantoea vagans cloned into pHD This study
pHD-RspB crtB from Rhodobacter sphaeroides cloned into pHD This study
pHD-PanI crtI from Pantoea ananatis cloned into pHD This study
pHD-PagI crtI from Pantoea agglomerans cloned into pHD This study
pHD-PvaI crtI from Pantoea vagans cloned into pHD This study
pYC1k-ccdB-idi p15A origin, Tac promoter, KanR, ccdB operon, idi This study
pSB1s-X pSC101 origin, Pbad promoter, StrR, dxs from Escherichia coli cloned into pSB1s Our lab