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Table 5 Oligos synthesized for lacZ assembly

From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method

Oligo Sequence
oligo1-1 F CTATaagcatcagacagcactg
oligo1-1R GTAAcagtgctgtctgatgctt
Oligo1-2 F TTGAagcttatcggatcgagcc
Oligo1-2R CGCCggctcgatccgataagct
Oligo3-1 F CTGAacggcaagc cgttgctga
Oligo3-1R CGAAtcagcaacggcttgccgt
Oligo3-2 F GGATttttgcatc gagctgggt
Oligo3-2R TATTAcccagctcgatgcaaaa
Oligo4-1 F TGACtacctacgg gtaacagtt
Oligo4-1R AAGAaactgttacccgtaggta
Oligo4-2 F GTTTacagggcgg cttcgtctg
Oligo4-2R GTCCcagacgaagccgccctgt
Oligo4-3 F GATTggcctgaac tgccagctg
Oligo4-3R GCGCcagctggcagttcaggcc
Oligo5-1 F TTGGagtgacggcagttatctg
Oligo5-1R CTTCcagataactgccgtcact
Oligo5-2 F GAGCgaacgcgta acgcgaatg
Oligo5-2R GCACcattcgcgttacgcgttc
Oligo5-3 F CTGAactaccgca gccggagag
Oligo5-3R GGCGctctccggctgcggtagt
Oligo5-4 F CGCGcgaattgaa ttatggccc
Oligo5-4R GTGTgggccataattcaattcg