Table 5 Oligos synthesized for lacZ assembly
From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method
| Oligo | Sequence |
|---|---|
| oligo1-1 F | CTATaagcatcagacagcactg |
| oligo1-1R | GTAAcagtgctgtctgatgctt |
| Oligo1-2 F | TTGAagcttatcggatcgagcc |
| Oligo1-2R | CGCCggctcgatccgataagct |
| Oligo3-1 F | CTGAacggcaagc cgttgctga |
| Oligo3-1R | CGAAtcagcaacggcttgccgt |
| Oligo3-2 F | GGATttttgcatc gagctgggt |
| Oligo3-2R | TATTAcccagctcgatgcaaaa |
| Oligo4-1 F | TGACtacctacgg gtaacagtt |
| Oligo4-1R | AAGAaactgttacccgtaggta |
| Oligo4-2 F | GTTTacagggcgg cttcgtctg |
| Oligo4-2R | GTCCcagacgaagccgccctgt |
| Oligo4-3 F | GATTggcctgaac tgccagctg |
| Oligo4-3R | GCGCcagctggcagttcaggcc |
| Oligo5-1 F | TTGGagtgacggcagttatctg |
| Oligo5-1R | CTTCcagataactgccgtcact |
| Oligo5-2 F | GAGCgaacgcgta acgcgaatg |
| Oligo5-2R | GCACcattcgcgttacgcgttc |
| Oligo5-3 F | CTGAactaccgca gccggagag |
| Oligo5-3R | GGCGctctccggctgcggtagt |
| Oligo5-4 F | CGCGcgaattgaa ttatggccc |
| Oligo5-4R | GTGTgggccataattcaattcg |
