Table 5 Oligos synthesized for lacZ assembly
From: A novel approach for metabolic pathway optimization: Oligo-linker mediated assembly (OLMA) method
Oligo | Sequence |
|---|---|
oligo1-1Â F | CTATaagcatcagacagcactg |
oligo1-1R | GTAAcagtgctgtctgatgctt |
Oligo1-2Â F | TTGAagcttatcggatcgagcc |
Oligo1-2R | CGCCggctcgatccgataagct |
Oligo3-1Â F | CTGAacggcaagc cgttgctga |
Oligo3-1R | CGAAtcagcaacggcttgccgt |
Oligo3-2Â F | GGATttttgcatc gagctgggt |
Oligo3-2R | TATTAcccagctcgatgcaaaa |
Oligo4-1Â F | TGACtacctacgg gtaacagtt |
Oligo4-1R | AAGAaactgttacccgtaggta |
Oligo4-2Â F | GTTTacagggcgg cttcgtctg |
Oligo4-2R | GTCCcagacgaagccgccctgt |
Oligo4-3Â F | GATTggcctgaac tgccagctg |
Oligo4-3R | GCGCcagctggcagttcaggcc |
Oligo5-1Â F | TTGGagtgacggcagttatctg |
Oligo5-1R | CTTCcagataactgccgtcact |
Oligo5-2Â F | GAGCgaacgcgta acgcgaatg |
Oligo5-2R | GCACcattcgcgttacgcgttc |
Oligo5-3Â F | CTGAactaccgca gccggagag |
Oligo5-3R | GGCGctctccggctgcggtagt |
Oligo5-4Â F | CGCGcgaattgaa ttatggccc |
Oligo5-4R | GTGTgggccataattcaattcg |