Fig. 6From: Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzymeQuantification of a isopimaradiene and b isopimaric acid detected in infiltrated leaves. The bar labelled (diTPS-ISO) indicates the quantities of isopimaradiene/isopimaric acid produced upon co-expressing diTPS-ISO, DXS and GGPPs constructs. All other bars display the quantities of isopimaradiene/isopimaric acid produced upon co-expressing different CYP constructs with DXS, GGPPs and diTPS-ISO. Isopimaradiene is quantified relatively to the internal standard eicosene, and the isopimaric acid quantity was quantified relative to plant dry weight (PDW). Biological triplicates were used for the quantificationBack to article page