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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

Fig. 6

Quantification of a isopimaradiene and b isopimaric acid detected in infiltrated leaves. The bar labelled (diTPS-ISO) indicates the quantities of isopimaradiene/isopimaric acid produced upon co-expressing diTPS-ISO, DXS and GGPPs constructs. All other bars display the quantities of isopimaradiene/isopimaric acid produced upon co-expressing different CYP constructs with DXS, GGPPs and diTPS-ISO. Isopimaradiene is quantified relatively to the internal standard eicosene, and the isopimaric acid quantity was quantified relative to plant dry weight (PDW). Biological triplicates were used for the quantification

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