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Table 1 PCR primers

From: Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

Name

Sequence (5'-3')

Comment

P1

TCGCGACCGGTAATGGCGCCCATGGC

CYP720B4 forward primer

P2

ATCTCGAGTTATTCATTCTCTACTCTACCATGAAG

CYP720B4 reverse primer

P3

GCGACCGGTAATGGCTCTGTTATTAGC

Barnes-CYP720B4 forward primer

P4

TCGCGACCGGTAATGAGTAATAAGGAGGT

ΔTm-CYP720B4 forward primer

P5

CGACCGGTAATGGCTTCCACTGCTC

Tp forward primer

P6

AGCGGCTGTGACACG

Tp reverse primer

P7

TCGTCTTTGTAGTCTTCATTCTCTACTCTAC

CYP720B4 reverse primer, containing FLAG tag