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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

Fig. 6

SERS immunoassay development. Fig. 6a presents a visual protocol for the development of a light scattering immunoassay. Step 1: Antigen is non-covalently bound to the polystyrene plate. Step 2: Buffer is used to remove unbound antigen. Step 3: The polystyrene surface is blocked to prevent non-specific binding. Step 4: SERS probe detection antibody is added to the plate. Step 5: Wash buffer is used to remove unbound SERS probe. Step 6: Light scattering from the SERS probe is measured for assay quantification. The resulting assay shows specific binding of the SERS probe to polystyrene embedded human IgG (Fig. 6b)

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Journal of Biological Engineering

ISSN: 1754-1611