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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

Fig. 7

AuNP and AuNR light scattering immunoassay with detection by optical absorbance spectroscopy. After antibody-conjugated gold nanoparticles were bound to the immunoassay plate, the assay response could be detected using optical absorbance spectroscopy. The absorbance spectrum of each well was recorded and the intensity of the major LSPR peaks for both AuNP and AuNR SERS probes was determined (Fig. 7a, c). The LSPR response was correlated to the nanoparticle concentration and a standard curve and sensitivity plot was developed (Fig. 7b, 7d). The curve was fit using five-parameter logistic equation. LLOD values for AuNP and AuNR assays detected using optical absorbance spectroscopy were 22.8 pM and 60.3 pM, respectively

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