Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

Table 1 SERS assay dynamic range

Nanoparticle Type	Raman Reporter	Dynamic Range	Lower Limit of Detection (LLOD)	Polystyrene Substrate?	Reference
Gold Nanorods (LSPR 815nm)	NIR Dyes	1 - 100 pM	1 pM	No	[33]
Silver Nanospheres Dimers	4-MBA	1 -500 pM	1 pM	No	[34]
Silica Coated Gold Nanospheres	S420, S421, S440	1-400 pM	1 pM	No	[35]
Silica Coated Gold Nanospheres	S420,S440	1-1200 pM	1 pM	No	[36]
Silica Coated Gold Nanospheres	S420, S421, S440	0.8 - 800 pM	0.8 pM	No	[37]
60 nm Gold Nanospheres	DTTC Iodide	76 - 500 pM	76 pM	Yes	**
Gold Nanorods (LSPR 779nm)	DTTC Iodide	45 - 500 pM	45 pM	Yes	**

The typical dynamic range for SERS-based immunoassays and cell labeling ranges from 1–1200 pM SERS probe [33–37]. The dynamic range is expressed as the actual number density of the SERS particles and should not be confused with the concentration of Raman reporter molecule added to the particles surface. Raman reporter concentration will typically range from 1,200 - 10,000 reporter molecules per colloid [82]. Note: ** SERS probe detection limits and Raman-based assay development methods presented in this paper

ISSN: 1754-1611