Fig 4
From: Modulating the import of medium-chain alkanes in E. coli through tuned expression of FadL
Effect of tightly-regulated expression of E. coli FadL and OmpW on the whole-cell bioconversion of octane. Specific maximum specific activity conditions in single well reactions from a multifactorial inducer study (Additional file 1: Figure S2) after 1 h overexpression of OmpW, FadL, and AlkL in the E. coli HB101 background strain from an optimized, L-rhamnose inducible vector at optimal inducer concentrations identified for each protein (10, 50, 500, and 1000 μM), compared with wild type strain. AlkB expression was pre-induced with DCPK, and wells were oxygenated and saturated with excess octane substrate (30 % v/v)