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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

Fig. 6

a Concentration of uAA-eGFP after 24 h in culture as a function of uAA concentration. Each column represents the mean + SD of four independent experiments (p < 0.001). b Comparison between protein concentrations of eGFP analogues as determined by ELISA and online measured fluorescence intensities of eGFP analogues. Data points illustrated as squares and labeled with Plk represent Plk-eGFP samples, whereas diamonds and circles display Alk-eGFP and Lys-eGFP, respectively. Tags indicate the uAA concentration in mM. All results are given as mean ± SD (n = 4) (c). Online measured fluorescence intensities of eGFP analogues in respect to uAA concentration. uAA and IPTG (433 μM) were added at the start of the cultivation

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Journal of Biological Engineering

ISSN: 1754-1611