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Table 1 Course schedule

From: Design and integration of a problem-based biofabrication course into an undergraduate biomedical engineering curriculum

Lab 1 (3 weeks)

Biocompatibility

Test the effect of different types of commonly used chemical compounds in 3D printers on cell viability

Lab 2 (3 weeks)

Lentiviral Transduction

Learn standard methods for delivering genes to cell lines and assessing transduction efficiency

Lab 3 (3 weeks)

3D Cell Culture in Hydrogel Scaffold

Culture muscle cells in 3D hydrogel scaffolds and quantify cellular adhesion, morphology, proliferation, and differentiation in 3D

Lab 4 (3 weeks)

Build a Walker Bio-Bot

3D print walking bio-bot powered by tissue engineered skeletal muscle and compute muscle force generation and bio-bot speed

Final Project (3 weeks)

Design and Build Your Own Bio-Bot

Design and build muscle-powered bio-bot to achieve a functional behavior relevant to an application in biomedical engineering