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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Design and engineering of a transmissible antiviral defense

Fig. 6

Dynamics with both phages. a, b Two replicates in which phage T5 was added to a culture of E. coli that had been infected and grown overnight with vaccine/phagemid and helper (dashed lines) or added to a culture that was not exposed to phagemid or helper (solid lines). (Vaccine and helper were added at a multiplicity of infection of 1.0 and 0.01, respectively, at initiation of the overnight culture). There is a sharp rise in T5 density after addition regardless of the presence of the vaccine (blue curves). However, cell density rebounds in the vaccine-protected population from growth of the survivors, which are resistant. Due to the effect of helper on cell growth, a higher initial density of cells is present in the unprotected culture than in the protected culture. c Drop in host density compared between presence and absence of the vaccine. The decline in cell density from 2hr to 4h is only 0.75 and 1.15 logs less when the vaccine is present than when it is absent (comparing a and b)

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